		Technical Data
C7510-19A	Collagen Type II

Description:
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemsitry (formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Cartilage in lung or fetus

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	0.N.226	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Chicken	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Purified type II collagen obtained from the adult chicken sternum by limited pepsinization. Epitope: triple helix. Cellular Localization: Cartilage matrix.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C7510-19AX.

Specificity:

Recognizes chicken Type II collagen. Species Crossreactivity: human, mouse and salamander.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Linsenmayer TF, Hendrix MJ. Monoclonal antibodies to connective tissue macromolecules: type II collagen. Biochem Biophys Res Commun 1980; 92(2):440-6 2. Mayne, R. et al., Connective Tissue Research 31(1):11-21 (1994).