Technical Data
C7510-20F
Collagen Type II
Description:
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Non-denaturing): 1:5000-:10,000
Standard ELISA: 1:4000-1:8000
Immunohistochemistry (Frozen): 1:50-1:200
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Collagen Type II from human knee cartilage and bovine nasal cartilage.
Purity:
Purified by immunoaf nity chromatography.
Form
Supplied as a liquid in 0.125M sodium borate, pH 8.0, 0.075M sodium chloride, 0.005M EDTA, 0.01% sodium azide.
Specificity:
Recognizes most mammalian Type II collagens and has negligible crossreactivity with Type I, III, IV, V and VI collagens. Nonspeci c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1. Chandra, M. et al., (2006) Opt Express. 14:6157-6171. 2. Liao, E. et al., (2007) Tissue Eng. 13:537-550. 3. Nicodemus, G. D. et al., (2011) Acta Biomaterialia 7; 492-504. 4. Skaalure, S.C. et al., (2012) Biomed. Mater. 7 024111 doi:10.1088/1748-6041/7/2/024111. 5. Farnsworth, N. et al. (2012) Osteoarthritis and Cartilage, http://dx.doi.org/10.1016/j.joca.2012.06.015.