Technical Data
C7510-20T
Collagen II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, COL2A1 Protein, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
immunohistochemistry (frozen): 1:10-1:50
immunohistochemistry (paraffin): 1:10-1:50
ELISA: 1:500-1:2,000
Epitope retrieval is recommended with 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37C and multiple buffer washes.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C for short-term only.Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul4C/-20C (No Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Human collagen type II from cartilage.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from mannitol, dextran, and salts. Reconstitute with 500ul sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes human Collagen type II.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.