		Technical Data
C7510-37F	COL2A1, NT (Collagen alpha-1(II) Chain, Alpha-1 Type II Collagen, Chondrocalcin)

Description:
Alpha-1 chain of type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutant forms of this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa476-505 from the N-terminal region of human COL2A1.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human COL2A1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Olavarrieta,L., Clin. Genet. 73(3), 262-267 (2008). 2.McAlinden,A., Hum. Mutat. 29(1), 83-90 (2008). 3.Forzano,F., Am. J. Med. Genet. A 143(23), 2815-2820 (2007).