Technical Data
C7510-44M
Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, EDS4A, Fetal Collagen, FLJ34534, Type III Collagen)
Description:
Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I. Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with aortic aneurysm abdominal (AAA).

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Dot Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
ELISA: Native human collagen III
Dot Blot: Native human collagen III
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Human kidney, liver, skin, heart

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Native human Collagen type III
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes human Collagen III. Minimal crossreactions with other collagens were only found if 20-50 times higher concentrations were used of other collagens.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amenta, et al., 1986, Collagen rel. res. 6, 125-152. 2. Rukosuev, et al., 1990, Histochem. 89, 11-16. 3. Shekhonin, et al., 1985, Collagen rel. res. 5, 355-368.