Technical Data
C7510-44N
Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, MGC93704, Type III Collagen)
Description:
Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I. Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with aortic aneurysm abdominal (AAA).

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
Immunocytochemistry: Cultured rat cells
ELISA: Native rat collagen III
Dot Blot: Native rat collagen III
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Rat kidney, skin

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRatRabbit
Concentration:
~0.1mg/ml
Immunogen:
Rat Collagen type III.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes rat Collagen III. Minimal crossreactions with other collagens were only found if 10-20 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.