Technical Data
Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base
The collagen family is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral selfassociation in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function, such as bone strength and platelet activation.

Suitable for use in ELISA, Dot and Slot Blot, Indirect Immunofluorescence and Indirect Immunohistochemistry. Not recommended on Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:3000
Immunofluorescence (Indirect): 1:20-1:40
Immunohistochemistry (Indirect): 1:20-1:40. Use frozen or acetone fixed specimens (paraffin not recommended)
Dot/Slot Blot: 1:300
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Purified human placental collagen type IV
Purified by affinity chromatography.
Supplied as a liquid in PBS, mannitol, dextran. No preservatives added.
Recognizes native and heat denatured human Collagen Type IV. Antiserum was passed over immobilized human blood plasma proteins and human collagen types I, III and V. Human plasma does not interfere with binding to collagen. Species Crossreactivity: collagen types I, III and V; <10%; human collagen II; <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Rennekampff, H.O. et al. (1996). Surgery. 120(1):1622. 2.Moulin, V. et al. (2000). Burn. 26:312. 3.Rukosuev VS et al. Acta Histochem 89(1):11-16