Technical Data
Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 2 Cha
Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin). Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:5-1:50
Immunohistochemistry (paraffin): 1:5-1:50
ELISA: 1:800-1:1,200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgM10B2288Highly Purified
1ml-20CBlue IceHumanMouse
Not determined
Native human collagen type IV.
Purified by Ion Exchange chromatography.
Supplied as a lyophilized powder from PBS, 0.1% mannitol, 0.1% dextran. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Recognizes human collagen type IV. Less than 2% crossreactivity observed with collagen types I, II, III and V.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.