		Technical Data
C7510-53C	Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3

Description:
The extent of expression of basement membrane component collagen IV may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. in diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum is important in the the differential diagnosis of bullous lesions in dermatopathology.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Acetone fixed frozen sections, formaldehyde fixed, pepsin pretreated paraffin sections): 1:50-1:100 using the Indirect method. Dilute immediately before use with PBS or Tris.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Serum

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Collagen IV from human placenta. Cellular Localization: Extracellular

Purity:

Serum

Form

Supplied as a liquid.

Specificity:

Recognizes human Collagen IV. Crossreactive with basement membrane of most species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Visser, R., et al., 1986, Histopathology 10, 171-180.