Technical Data
Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antibody is important in the differential diagnosis of bullous lesions in dermatopathology.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: Exclusively reactive with basal membranes.
Immunohistochemistry (Frozen, paraffin): 1:10-1:20 using the indirect method. Dilute immediately before use with PBS. Fixation with acetone for 5 min. at -20C is recommended for frozen sections. Paraffin material should be fixed with neutral formalin. For such tissue sections proteolytic pretreatment with pepsin is recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
Skin, kidney

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceHumanMouse
Not determined
Collagen IV isolated from human placenta. Cellular Localization: Extracellular basement membranes
Supplied as a liquid in 0.15M PBS, 1% BSA, 0.02% sodium azide.
Recognizes human collagen type IV. Does not crossreact with any other collagen types.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Havenith, M.G., et al., 1987, Histochemistry, 87, 123-128.