Technical Data
C7510-53L
Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 2 Cha
Description:
Collagen type IV is a major componant of basement membranes, such as those associated with capillaries and within glomeruli. This antibody will help detect col- logen type IV and determine the presence of an intact basal lamina in normal tissues or the loss of structure as seen in various carcinomas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:200 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Kidney (basement membranes staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1PHM-12Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human glomeruli.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Specificity:
Recognizes human Collagen Type IV.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gutterson B A, et al.. Invading squamous cell carcinoma can retain a basal lamina. Laboratory Investigation. 51:82-87 (1984). 2.Hancock W W, et al.. Production of monoclonal antibodies to fibronectin, type IV collagen and other antigens of the human glomerulus. Pathology. 16:197-206 (1984).