		Technical Data
C7510-53L	Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 2 Cha

Description:
Collagen type IV is a major componant of basement membranes, such as those associated with capillaries and within glomeruli. This antibody will help detect col- logen type IV and determine the presence of an intact basal lamina in normal tissues or the loss of structure as seen in various carcinomas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:200 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Kidney (basement membranes staining pattern)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	PHM-12	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human glomeruli.

Purity:

Supernatant

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

Specificity:

Recognizes human Collagen Type IV.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gutterson B A, et al.. Invading squamous cell carcinoma can retain a basal lamina. Laboratory Investigation. 51:82-87 (1984). 2.Hancock W W, et al.. Production of monoclonal antibodies to fibronectin, type IV collagen and other antigens of the human glomerulus. Pathology. 16:197-206 (1984).