Technical Data
Columbia Broth (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone5.0
Peptic Digest of Animal Tissue5.0
Sodium Chloride5.0
Yeast Enriched Peptone10.0
Tryptic Digest of Heart Muscle3.0
Magnesium Sulfate0.1
Tris Aminomethane0.8
Ferrous Sulfate0.02
Tris Aminomethane HCl2.9
Sodium Carbonate0.6
Formulated to enhance recovery of aerobic and anaerobic organisms from blood cultures.

Eller et al. first described Columbia Broth. It was designed to produce rapid and luxuriant growth of a range of organisms due to its high nutrient content. It contains meat peptone, casein peptone and yeast extract all of which are highly nutritious components. The meat peptone contains sufficient sulfonamide antagonists to prevent sulfonamide inhibition of growth. Columbia Broth, in the presence of CO2 and supplemented with SPS, is an excellent blood culture medium.

Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Directions: 1. Dissolve 35grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi) Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Bacteroides fragilis ATCC 25285

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Amber, clear, complete.

pH (25C): 7.30.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.