Technical Data
COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH, Pseudoachondroplasia, THBS5, Thrombospondin 5)
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110kD modular matricellular protein. COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via integrins alpha5beta1 and alphaVbeta3. COMP is upregulated in inflammation and some carcinomas.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml detects human COMP in cell or tissue extracts.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human COMP from conditioned cell culture media.
Optimal dilutions to be determined by the researcher.

Western Blot Buffers:
Blotting Buffer: 25mM Tris, pH 7.4, 0.15M sodium chloride, 0.05% Tween-20
Blocking Solution: 5% nonfat dry milk in Blotting Buffer
Antibody Solution: 5% nonfat dry milk in Blotting Buffer

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG19H100Affinity Purified
100ug-20CBlue IceHumanMouse
Bacterially-expressed recombinant human Cartilage Oligomeric Matrix Protein (recombinant human COMP; Accession #P49747).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human COMP at ~110kD. Species Sequence Homology: rat, mouse, equine, bovine, canine (86-93%)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.