Technical Data
C7849-09A
COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH, Pseudoachondroplasia, THBS5, Thrombospondin 5)
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Technical Data |
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C7849-09A |
COMP (Cartilage Oligomeric Matrix Protein Precursor, EDM1, EPD1, Epiphyseal Dysplasia 1, Epiphyseal Dysplasia 1 Multiple, MED, MGC13181, MGC149768, PSACH, Pseudoachondroplasia, THBS5, Thrombospondin 5) |
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Description: COMP is a non-collagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:200-1:1000 Immunohistochemistry: 1:10-1:2000 Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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