Technical Data
C7849-09E
COMP, ID (Cartilage Oligomeric Matrix Protein, Thrombospondin-5, TSP5)
Description:
COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations.

Applications:
Suitable for use in ELISA, Western Blot. Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 314-343 in the central region of human COMP, UniProt Accession #P49747.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human COMP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim,H.J., et.al., Eur. J. Appl. Physiol. 105(5), 765-770 (2009).