Technical Data
C7850-08F
Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor)
Description:
Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognized by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry: 1:10
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19G173Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human C1s.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). C1s is capable of inhibiting C4 binding and activation.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Matsumoto, M et al; Probing the C4-binding site on C1s with monoclonal antibodies, evidence for a C4/C4b-binding site on the gamma-domain. J Immunol 1989, 8: 2743 2.Nakagawa, K et al; Complement C1s activation in degenerating articular cartilage of rheumatoid arthritis patients: immunohistochemical studies with an active form specific antibody. Ann Rheum Dis 1999, 58: 175