Technical Data
C7850-08G
Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor)
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Technical Data |
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C7850-08G |
Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor) |
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Description: Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognized by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10 Western Blot: 1:10 Immunohistochemistry: 1:10 Immunoprecipitation: 1:10 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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