Technical Data
C7850-13T9
C3ar1 (C3a Anaphylatoxin Chemotactic Receptor, C3AR, C3a-R)
Description:
In the course of complement activation the C3a receptor functions as the cell surface receptor for the anaphylatoxin C3a, the C-terminal 77aa cleavage product of the alpha chain of C3, but not for C3a-desArg. All cellular responses to C3a are specifically mediated by interactions with the membrane bound C3a receptor, a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 54kD C3a receptor is widely distributed in myeloid cells, peripheral tissues and the central nervous system. Expression of C3aR has been demonstrated on neutrophils, monocytes, eosinophils, astrocytes, neurons and glial cells and is increased in inflammatory conditions. No C3aR expression was detectable on lymphocytes and on tonsillar B cells even after stimulation.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:50
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG113A35Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes rat receptor for C3a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Luhmann, A et al; Immunohistochemical analysis of human and rat C3a receptor expression by the use of monoclonal antibodies. Mol Immunol 2001, 38: 108.