		Technical Data
C7850-18D	Complement C4d

Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:10-1:40
Immunohistochemistry (Paraffin): 1:10-1:40. Heat induced epitope retrieval is recommended.
Immunofluorescence: 1:10-1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Synthetic peptide corresponding to aa1241-1256 of C4 conjugated to Keyhole Limpet Hemocyanin (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes human complement split product C4d.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Regele, H. et al. (2001) Endothelial C4d deposition is associated with inferior kidney allograft outcome independently of cellular rejection. Nephrol. Dial. Transplant 16: 2058-2066.