Technical Data
C5, NT (Complement C5, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 4, Complement C5 beta Chain, Complement C5 alpha Chain, C5a Anaphylatoxin, Complement C5 alpha' Chain, CPAMD4)
C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As Reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 253-281 in the N-terminal region of human C5, UniProt Accession #P01031.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human C5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McClure,A.,, Rheumatology (Oxford) 48(11), 1369-1374 (2009). 2. DiScipio,, J. Biol. Chem. 267(24), 17087-17094 (1992).