Technical Data
C7850-24B
C5, NT (Complement C5, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 4, Complement C5 beta Chain, Complement C5 alpha Chain, C5a Anaphylatoxin, Complement C5 alpha' Chain, CPAMD4)
Description:
C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human C5 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human C5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. McClure,A., et.al., Rheumatology (Oxford) 48(11), 1369-1374 (2009). 2. DiScipio,R.G.et.al., J. Biol. Chem. 267(24), 17087-17094 (1992).