Technical Data
Complement C9 (CT) (FITC)
Human C9 is a soluble glycoprotein of 61kD. It is the last component in the assembly of the membrane attack complex (MAC). When the complement is activated on target membranes, multiple copies of C9 bind to the C5b-8 complex and assemble the barrel-shaped pore which causes cell lysis. The conformation of C9 changes from globular to a tubular form. The binding of C9 to C5b-8 plays a key role in the function of C9.

Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunohistochemistry and Inhibition. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:10
Flow Cytometry: 1:10
Immunohistochemistry (Frozen/paraffin): 1:10
Inhibition: Inhibits C9 binding to C5b-8.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG19G177Affinity Purified
100ug-20CBlue IceHumanMouse
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Recognizes human Complement C9 at ~61kD. Reacts with the C-terminal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hatanaka, M et al; Analysis of C5b-8 binding sites in the C9 molecule using monoclonal antibodies: participation of two separate epitopes of C9 in C5b-8 binding. Mol Immunol 1992, 29: 911