Technical Data
C7850-60G
CFB, ID (Complement Factor B, C3/C5 Convertase, Properdin Factor B, Glycine-rich beta Glycoprotein, GBG, PBF2, Complement factor B Ba fragment, Complement factor B Bb fragment, BFD, BF)
Description:
BF is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Center region of human BF (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CFB.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Baatz,H., Klin Monatsbl Augenheilkd 226(8), 654-658 (2009). 2. Goring,K., Am. J. Physiol., Cell Physiol. 296(5), C1140-C1150 (2009).