Technical Data
C7850-60H
CFB, ID (Complement Factor B, C3/C5 Convertase, Properdin Factor B, Glycine-rich beta Glycoprotein, GBG, PBF2, Complement factor B Ba fragment, Complement factor B Bb fragment, BFD, BF)
Description:
CFB encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (FFPE): 1:50-1:100
Flow Cytometry: 1:10-1:50
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 469-494 in the central region of human CFB, UniProt Accession #P00751.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human CFB. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Munch,I.C., et.al., Invest. Ophthalmol. Vis. Sci. (2009) In press. 2. Gateva,V., et.al., Nat. Genet. 41(11), 1228-1233 (2009).