Technical Data
Complement Factor D
Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml; Recombinant human Complement Factor D/Adipsin
Immunoprecipitation: 0.25ug/ml; Conditioned cell culture medium spiked with Recombinant human Complement Factor D/Adipsin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
~0.2mg/ml (after reconstitution)
Mouse myeloma cell line NS0-derived recombinant human Complement Factor D Ile26-Ala253, UniProt # P00746
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder from PBS, Trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human Complement Factor D in direct ELISAs and Western Blots. In direct ELISAs, less than 1% crossreactivity with recombinant human (rh) Complement Factor B and rhComplement Factor I is observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. White, R.T., et al., J. Biol. Chem. 267: 9210 (1992). 2. Taylor, F.R., et al., Biochemistry 38: 2849 (1999). 3. Kim, S., et al., J. Biol. Chem. 270: 24,399 (1995).