Technical Data
C7850-70E
Complement Factor D (Biotin)
Description:
Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3. Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Sandwich ELISA:
Capture (C7850-70C): 2-8ug/ml
Detection(C7850-70E): 0.1-0.4ug/ml
Western Blot: 0.1ug/ml using Recombinant human Complement Factor D/Adipsin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to aa26-253 from human Complement Factor D expressed in NSO cells (P00746).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Specificity:
Recognizes human Complement Factor D in ELISAs and Western Blots. In sandwich immunoassays, <0.1% crossreactivity with rhFactor II, rhFactor X and rhFactor XI.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. White, R.T., et al., J. Biol. Chem. 267: 9210 (1992). 2. Taylor, F.R., et al., Biochemistry 38: 2849 (1999). 3. Kim, S., et al., J. Biol. Chem. 270: 24,399 (1995).