		Technical Data
C7850-82A	Complement Factor H (CFH, H Factor 1, HF, HF1, HF2, beta1 H-globulin, b1H Globulin)

Description:
Factor H is a regulatory factor of the alternative complement pathway. Factor H is a glycoprotein consisting of a single polypeptide chain with molecular mass of 155kD. Human plasma concentration is approximately 500ug/ml.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.03-0.1ug/ml
ELISA: >1:32,000
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-HLVPDRKKDQYK, from the internal region of the protein sequence according to NP_000177.2;.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human Complement factor H.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Klein RJ, Zeiss C, Chew EY, Tsai JY, Sackler RS, Haynes C, Henning AK, Sangiovanni JP, Mane SM, Mayne ST, Bracken MB, Ferris FL, Ott J, Barnstable C, Hoh J. Complement Factor H Polymorphism in Age-Related Macular Degeneration. Science. 2005 Mar 10; [Epub ahead of print] PMID: 15761122.