Technical Data
C7853-02
Connexin 26, 32, 43 Antibody Array Kit, BioAssay™
1Kit
Kits and Assays Storage: 4°C (-20°C Glycerol)Shipping: Blue Ice
The Connexin gene family encodes the protein subunits of gap junction channels. The gap junction channels permit ions, nucleotides, and small molecules up to 1200 daltons to pass between cells. Gap junctions have been suggested to be involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control, and
developmental regulation.

Kit Components:
C7853-02A Connexin-26 Pab 1x10ug 0.25mg/ml
C7853-02B Connexin-32 Pab 1x10ug 0.25mg/ml
C7853-02C Connexin-43 Pab 1x10ug 0.25mg/ml
C7853-02D Connexin-26 Mab IgG1-kappa 1x20ug 0.5mg/ml
C7853-02E Connexin-32 Mab IgG1,k 1x20ug 0.5mg/ml
C7853-02F Connexin-43 Mab IgG1,k 1x20ug 0.5mg/ml
C7853-02G Connexin-26 and -32 Control Lysate Mouse Liver 1x50ul For C7853-02A, C7853-02B, C7853-02D and C7853-02E
C7853-02H Connexin-43 Cell Lysate For C7853-02C and C7853-02F.

Antibodies:
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Controls:
Supplied as a liquid in SDS-PAGE buffer (60mM Tris buffer, 2% SDS, 1% DTT, 0.003% bromophenol blue, 5% glycerol, pH 6.8)--do not boil.

C7853-02A, -02B, -02C are purified from ascities. C7853-02D, -02E, -02F are affinity purified from serum.
Antibodies C7853-02A, C, D, F recognize human connexin. AntibodiesC7853-02A-F recognize mouse and rat connexin.

Applications:
C7853-02A, B, D, E, F are suitable for use in ELISA, Immunohistochemistry and Western Blot.
C7853-02C is suitable for use in Immunoprecipitation, ELISA, Immunohistochemistry and Western Blot.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Immunohistochemistry: 1-5ug/ml
Immunoprecipitation: 1-5ug
Western Blot: 0.5-1ug/ml
Controls:
C7853-02G:15ul per lane for C7853-02A and C7853-02D on Nitrocellulose membrane.
C7853-02G: 5ul per lane for C7853-02A and C7853-02D on PVDF membrane.
C7853-02H:20ul per lane for C7853-02C
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing of antibodies, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquot controls and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity: Antibodies C7853-02A, C, D, F recognize human connexin.
Antibody C7853-02A-F recognize mouse and rat connexin.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1 Dahl, E., et al; J. Biol Chem, 271:17903-17910 (1996) 2. Nagy, J.I., et al; Neuroscience 78(2):533-548 (1997) 3. Nagy, J.I., et al; Neuroscience 88(2):447-468 (1999) 4. Kelsell, D.P., et al; Nature 387:80-83 (1997) 5. Hosny S. and Jennes L.; Neuroendocrinology 67:101-108 (1998) 6. Bittman, K.S., et al; Cerebral Cortex 9:188-195 (1999) 7. Kamibayashi, Y. et al; The Journal of Investigative Dermatology 101(6):773-778 (1993) 8. Martin, P.E.M. et al; J. Biol. Chem. 273(3):1719-1726 (1998) 9. Masgrau-Peya, E. et al; J. Histochem. Cytochem. 45(9):1207-1215 (1997) 10. Koval, M., et al; J. Cell Biol. 137(4):847-857 (1997)11. Toyofuku, T. et al; J. Biol. Chem. 273(3):1519-1528 (1998) 12. Xie, H., et al; J. Cell Biol. 137:203-210 (1997)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.