Technical Data
C7853-04
Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)
Description:
Intercellular communication through gap junctions plays an important role in a variety of cellular processes including
homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that
serve to directly link neighboring cells by mediating the exchange of low-MW <1200 kD metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. The
connexon itself is composed of a hexameric assembly of connexin proteins. Connexins are highly homologous proteins
encoded by a multigene family. The connexins exhibit similar structural features, which include a cytoplasmic amino terminal
region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1.0ug/ml
Immunohistochemistry (Rat): 10-20ug/ml
Western Blot (Mouse and Rat): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to 13aa derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single aa and from the human sequence by two (non-consecutive) amino acids.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Connexin 26. Species Crossreactivity: rat. Reactivity was confirmed by Western Blot using lysates derived from mouse liver, rat liver and rat brain. Species Sequence Homology: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, M. and Gilula, M.B., Cell 84:381-388 (1996). 2. Saez, J.C., et al; In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al; Neuron 6:305-320 (1990). 4. Kuraoka, A., et al; J. Histochem. and Cytochem. 41:971-980 (1993). 5. Wilgenbus, KK., et al; Int. J. Cancer 51:522-529 (1992). 6. Dahl, E. et al., J. Biol. Chem. 271:17903-17910 (1996). 7. Nagy, J.I., et al., Neuroscience 78:533-548 (1997).