		Technical Data
C7853-04	Connexin 26 (CX26, Gap Junction Beta 2 Protein, CXB-2)

Description:
Intercellular communication through gap junctions plays an important role in a variety of cellular processes including
homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that
serve to directly link neighboring cells by mediating the exchange of low-MW <1200 kD metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. The
connexon itself is composed of a hexameric assembly of connexin proteins. Connexins are highly homologous proteins
encoded by a multigene family. The connexins exhibit similar structural features, which include a cytoplasmic amino terminal
region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA (Immunogen): 0.1-1.0ug/ml
Immunohistochemistry (Rat): 10-20ug/ml
Western Blot (Mouse and Rat): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide corresponding to 13aa derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single aa and from the human sequence by two (non-consecutive) amino acids.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes mouse Connexin 26. Species Crossreactivity: rat. Reactivity was confirmed by Western Blot using lysates derived from mouse liver, rat liver and rat brain. Species Sequence Homology: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kumar, M. and Gilula, M.B., Cell 84:381-388 (1996). 2. Saez, J.C., et al; In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al; Neuron 6:305-320 (1990). 4. Kuraoka, A., et al; J. Histochem. and Cytochem. 41:971-980 (1993). 5. Wilgenbus, KK., et al; Int. J. Cancer 51:522-529 (1992). 6. Dahl, E. et al., J. Biol. Chem. 271:17903-17910 (1996). 7. Nagy, J.I., et al., Neuroscience 78:533-548 (1997).