Technical Data
C7853-06
Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)
Description:
Connexin 31 (Cx31) is a 270 amino acid gap junction protein in mouse and rat with a predicted molecular weight of ~31kD.1 Connexin 31 is encoded by the gene Gjb3, and is expressed in a complex pattern throughout mouse development. During early mouse development, Cx31 is present in both the inner cell mass and the trophectoderm, but it is restricted to cells derived from the trophectoderm lineage after implantation. In adult female rodents, Cx31 is expressed in the granular layer of the placenta, and the lack of Cx31 leads to transient dysmorphogenesis affecting embryonic survival. In adult male rodents, Cx31 is expressed in the seminiferous epithelium of testis and may play a role in cell-cell communication during spermatogenensis.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1-3ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 1-3ug/ml on fixed and paraformaldehyde-perfued tissues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of mouse connexin 3 protein.
Purity:
Purified by Immunoaffinity chromatgraphy.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the ~32kD mouse connexin 31 on Western Blots. The band observed at ~62kD is probably due to dimerization of connexin 31. Several higher bands of unknown origin are observed in Western Blots of mouse skin homogenates. Reactivity has been confirmed with mouse skin homogenate.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hennemann, et al. Eur J Cell Biol 57:51-58, 1992. 2. Dahl, et al. J Cell Sci 109(Pt 1):191-197, 1996. 3. Plum, et al. Dev Biol 231:334-347, 2001. 4. Mok, et al. FEBS Lett 453:243-248, 1999.