		Technical Data
C7853-40A	Connexin 30.3, NT (Gap Junction beta-4 Protein, Connexin-30.3, Cx30.3, GJB4)

Description:
Gap junctions permit direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular communication. Gap junction channels consist of connexin protein subunits encoded by a multigene family. Erythrokeratodermia variabilis (EKV) is an autosomal dominant disorder of keratinization characterized by migratory erythematous lesions and fixed keratotic plaques. Mutations in the GJB3 gene have been reported in some but not all families, although it has been postulated that the absence of connexin 30.3 can be compensated by other connexins.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human GJB4 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human GJB4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). 2. Richard, G., et al., J. Invest. Dermatol. 120(4):601-609 (2003). 3. Macari, F., et al., Am. J. Hum. Genet. 67(5):1296-1301 (2000). 4. Lopez-Bigas, N., et al., Hum. Mutat. 19(4), 458 (2002).