Technical Data
C7853-41
Connexin 30.3 (CX30.3, Gap Junction Beta 5 Protein, CXB5)
Description:
Connexin 30.3 (Cx30.3) is expressed in the rat kidney, the theca interna and the granulose cell compartment of the porcine ovarian follicle. Mutation of the gene for Cx30. has been tied to Erythrokeratodermia Variabilis (EKV), an autosomal dominant keratinization disorder characterized by migratory erythematous lesions and fixed keratotic plaques. Mutations in Cx30.3 are also involved in hearing impairment.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 2ug/ml
Western Blot: 1-3ug/ml
Immunofluorescence: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the internal region of the mouse connexin 30.3 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the ~30kD mouse connexin 30.3 protein. Bands of unknown origins are observed at ~45kD, ~60kD, and between 70-90kD. Reactivity has been confirmed with mouse skin homogenates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tucker MA, et al. Exp Cell Res 213:224-230, 1994. 2. Itahana K, et al. Endocrinology 137: 5036-44, 1996. 3. Macari F, et al. Am J Hum Genet 67: 1296-1301, 2000. 4. Plantard L, et al. Hum Mol Genet 12:3287-3294, 2003. 5. Richard G, et al. J invest Dermatol 120:601-609, 2003. 6. Lopez-Bigas N, et al. Hum Mutat 19:458, 2002.