Technical Data
C7853-42
Connexin 30.3 (CX30.3, Gap Junction Protein, Beta 4 GJB4)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL

ELISA: 1:100,000 using 50-100ng control peptide/well.

Optimal dilutions to be determined by the researcher.

Control Peptide: C7853-44

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
As reported
Immunogen:
A 20 amino acid peptide sequence within the first cytoplasmic domain of rat Cx30.3. The peptide was coupled with KLH (carrier protein) using NH2-terminal Cysteine. The peptide sequence is identical in mouse Cx30.3 and has no significant homology with othe
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Specificity:
Recognizes rat Cx30.3 (predicted MW: 31kD). Actual crossreactivity of antibodies in various species is not known.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; Evans, HW (1994) Biocem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194
2. Tucker, MA and Barajas, L (1994) Exp. Cell Res. 213, 224, 230;;
3. Hennemann, H