Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1-10ug/ml using ECL
ELISA: 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide: C7853-44
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|A 20 amino acid peptide sequence within the first cytoplasmic domain of rat Cx30.3. The peptide was coupled with KLH (carrier protein) using NH2-terminal Cysteine. The peptide sequence is identical in mouse Cx30.3 and has no significant homology with othe|
|Purified by immunoaffinity chromatography|
|Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.|
|Recognizes rat Cx30.3 (predicted MW: 31kD). Actual crossreactivity of antibodies in various species is not known.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; Evans, HW (1994) Biocem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194|
2. Tucker, MA and Barajas, L (1994) Exp. Cell Res. 213, 224, 230;;
3. Hennemann, H