Technical Data
C7853-50
Connexin 29
Description:
Gap junctions have important functions in the transmission of signals across synapses in the nervous system (3). Connexin29 is a gap junction protein of 258 amino acids encoded by a novel single copy mouse gene that shows 75% sequence identity to human connexin 30.2 (4). Connexin29 mRNA (4.4 kb) is highly abundant in mouse sciatic nerve and is less abundant in spinal cord and adult brain (4). Expression data suggest that the new connexin gene is active in myelin-forming glial cells (4).

Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain or sciatic nerve homogenates

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
0.25mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal sequence of mouse Cx29.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4 0.01% sodium azide.
Specificity:
Recognizes mouse Cx29. Peptide inhibition studies were used to con rm the antibody’s speci city. In Western Blot, two bands are usually observed. A band at ~29 kD is the connexin29 monomer, while a second band at ~58 kD corresponds to the Cx29 dimer.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Balice-Gordon R J. et al. J. Cell Biol. 142:1095-1104 (1998). 2. Nagy et al., under preparation. 3. Rozental et al. Brain Res Brain Res Rev. 32: 11-15 (2000). 4. Sohl G et al. Biol. Chem. 382: 973-978 (2001).