Technical Data
C7853-60
Connexin 30 (Cx30)
Description:
Connexin 30 (Cx-30) is a newly identified member of the connexin gene family. Cx-30 was isolated by screening a genomic mouse library with a rat Cx26 probe (10). The Cx30 protein is most closely related to the Cx26 protein (77% amino acid sequence identity) (10,11). The expression patterns of Cx30 and Cx26 are clearly distinct, specifically, Cx30 is highly expressed in adult skin and brain; however, it cannot be detected in the embryonic and fetal brain (10,11). On the other hand, Cx26 is expressed highly in prenatal brain and decreases after birth.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1.0ug/ml
Western Blot (mouse and rat): 1ug/ml; Detects a protein of ~30kD corresponding to the authentic Connexin 30 (Cx30) protein. A Cx30 dimer is not detected although other unidenti ed bands migrating between 70-80kD may be observed. When used to probe Western Blots of rat liver (rat liver does not contain Cx30), several unknown bands are detected at ~110, 53 and 35kD.
Immuno uorescence (frozen sections): 1-5ug/ml
Immunohistochemistry (frozen): Immunostaining of xed, frozen adult rat brain with C7853-60 produces a typical punctate staining pattern in the globus pallidus/hypothalamus. It is not determined if the antibody is reactive in IHC with mouse samples.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3G102Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to the unique C-terminus of mouse Connexin 30.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Connexin 30. Detects a protein of ~30kD corresponding to the authentic Connexin 30 (Cx30) protein. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Saez, J.C., et al., In Advances in Second Messenger and Phosphoprotein Research (1993). 2. Bennet, M.V.L., et al., Neuron 6: 305-320 (1990). 3. Beyer, E., et al., J. Cell. Biol. 105: 2621-2629 (1987). 4. Beyer, E.C., J. Cell Biol. 108: 595-605 (1989). 5. Crow, D.S., et al., Mol. Cell. Biol. 10: 1754-1763 (1990). 6. Fishman, G.I., et al., J. Cell. Biol. 111: 589-598 (1990). 7. Kanter, H.L., et al., Circ. Res. 73: 344-350 (1993). 8. Wilgenbus, K.K., et al., Int. J. Cancer 51: 522-529 (1992). 9. Davis, L.M., et al., J. Amer. Coll. Cardiol. 24: 1124-1132 (1994). 10. Dahl, E., et al., J. Biol. Chem. 271: 17,903-17,910 (1996). 11. Nagy, J.I., et al., Neuroscience 78: 533-548 (1997). 12. Rash, J.E., et al., J. Neuroscience 21: 1983-2000 (2001).