Technical Data
Connexin 31.1 (CX31.1.1, Gap Junction Beta 5 Protein, CXB-5)
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for westerns (3).

ELISA (1:100K using 50-100ng CX31.1 control peptide/well.
Immunoprecipitations. We recommend the use of affinity pure antibodies at 2-10ug affinity pure antibodies. An antibody made to a similar epitope has been used for Immunoprecipitations (3). A similar protocol is recommended for this antibody.

Immunohistochemistry & Immunofluorescence: we recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue (3).

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
As reported
A 14 amino acid peptide sequence within the 3rd cytoplasmic domain of the rat Cx31.1 (2) was used to generate anti-rat Cx31.1. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
Supplied as a liquid, neat serum, 40% glycerol.
The Cx31.1 immunogenic peptide sequence is 86% (12/14 aa) homologous with mouse CX31.1 (4). The Cx31.1 peptide sequence does not have significant homology with other known Connexins. Actual crossreactivity of antibodies in various species is not established
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; White , WT et al (1995) Kidney Intl. 48, 1148-1157; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194
2. Haefliger, JA et al (1992) J. Biol. Chem. 267, 2057
3. Goliger, J et al (1994) Dev. Dynamics 200, 1-13
4. Hennemann, H et al (1992) J. Biol. Chem. 267, 17225-17233