Technical Data
C7853-61E
Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3).
ELISA: 1:100K using 50-100ng CX31 control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
C7853-61D Connexin 31, Mouse, Control Peptide (CX31, Gap Junction Beta 3 Protein, CXB-3).

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
A 17 amino acid peptide sequence within the 2nd cytoplasmic domain of the rat Cx31 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes mouse Connexin 31. Species sequence homology: shows 3 aa differences in rat Cx 31. The Cx31 peptide sequence does not significant homology with other known Connexins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Citations:
1. Fischer R, 2005 Gastroenterology, 128, 433-448 Intercellular communication via gap junctions in activated rat hepatic stellate cells. WB, IHC. 2. Schenke LK 2007 Stem Cells. 2007 Jun;25(6):1529-38. Epub 2007 Mar 15. Collagen IV induces trophoectoderm differentiation of mouse embryonic stem cells. IHC.

1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; 2. Evans, HW (1994) Biochem. Soc. Tr. 788-792; 3. Byer, E et al (1990) J. membrane Biol. 116, 187-194. 4. Henneman, H et al (1992) Eur. J. Cell Biol. 57, 51-58 5. Butterweck, A et al (1994) Eur. J. cell Biol. 65, 152-163 6. Hoh, JH et al (1991) J. Biol. Chem. 266, 6524-6531