		Technical Data
C7853-63	Connexin 31 (Connexin-31, CX31, DFNA2, DFNA2B, EKV, FLJ22486, Gap Junction Beta 3 Protein, Gap Junction Protein beta 3 31kDa, GJB3, MGC102938) (Azide Free)

Description:
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.

Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	10B2574	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant protein corresponding to aa1-271 of human Connexin 31.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human CONNEXIN 31.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.