		Technical Data
C7854-01D	Connexin 32, NT (Gap Junction beta-1 Protein, Connexin-32, Cx32, GAP Junction 28kD Liver Protein, GJB1)

Description:
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels.GJB1 is normally found in the paranodal myelin loops and Schmidt-Lanterman incisures of myelinating Schwann cells in the peripheral nervous system, as well as in oligodendrocytes and their processes, but not in compact myelin of the central nervous system. A series of mutational and other studies have directly linked defects in GJB1 to X-linked Charcot-Marie-Tooth disease (CMTX), a clinically and genetically heterogeneous group of hereditary motor and sensory peripheral neuropathies.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human GJB1 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human GJB1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Yang, J., et al., Biochem. Biophys. Res. Commun. 307(1):80-85 (2003). 2. Sahin, N., et al., Int. J. Neurosci. 113(6):777-785 (2003). 3. Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003). 4. Takashima, H., et al., Acta Neurol Scand 107(1):31-37 (2003). 5. Govindarajan, R., et al., J. Biol. Chem. 277(51):50087-50097 (2002).