Technical Data
C7854-02
Connexin 32 (CX32, C’B1, Gap Junction Beta1 Protein, Gap Junction Liver Protein)
Description:
Applications:
Suitable for use in Immunohistochemistry (lightly formaldehyde fixed tissues) and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western blot: Detects a 27kD Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47kD band corresponding to aggregates.

Optimal dilution to be determined by the researcher.

Hybridoma:
Produced by the immunization of BALB/c mice with isdolated rat junctional complexes.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2Q252Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
Purity:
Purified IgG
Form
Supplied as a liquid in PBS, 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes rat Connexin-32. Species crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Goodenough, et al. (1998) J. Cell Biol. 107, 1817.