Technical Data
C7854-05
Connexin 32
Description:
Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of lowmolecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length(1,2) Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane(3). Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis(3,4).

Applications:
Suitable for use in Immunofluorescence, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 10ug/ml
Western Blotting: 1-3ug/ml
Immunohistochemistry: 10ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3G104Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
0.5mg/ml
Immunogen:
Synthetic peptide derived from the C-terminus of mouse
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Reacts with both the monomer and dimer forms of Connexin 32 (molecular weights of ~32kD and 51kD). Reactivity is con rmed with brain and liver from rat and mouse. Based on sequence homology, this antibody is expected to react with human Connexin 32.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beyer, E., et al; Cell. Biol. 105:2621-2629 (1987).
2. Fishman, G.I., et al; J. Cell. Biol.. 111:589-598 (1990).
3. Crow, D.S., et al; Mol. Cell. Biol. 10:1754-1763 (1990).
4. Wilgenbus, et al; Int. J. Cancer 51:522-529 (1992).