Technical Data
C7854-05D
Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)
Description:
Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against this epitope has been used for Westerns (3) to detect a broad band of 28-30kD. Additional high MW. bands (aggregated Cx32) may also be detected.

ELISA (1:100K using 50-100ng Cx32 control peptide/well.

Immunohistochemistry & Immunofluorescence: We recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue as described (3).

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
As reported
Immunogen:
A 19 amino acid peptide sequence within the 2nd cytoplasmic domain of the rat Cx32 (2) was used to generate anti-rat Cx32. Amino terminal Cysteine was added for conjugation to KLH (carrier protein)
Purity:
Serum
Form
Supplied as a liquid, neat serum, 40% glycerol.
Specificity:
Rat Cx32 immunogenic peptide sequences is the same in mouse, rat and human (4
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; White , WT et al (1995) Kidney Intl. 48, 1148-1157; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194
2. Paul D (1986) J Cell Biol. 103, 123-134; Heynkes, R et al (1986) FEBS Lett. 205, 56-60; Nicholson, B (1985) JBC 260, 6514; Zimmer, DB et al (1987) JBC 262, 7751; Rahman, S et al (1991) J Cell Sci. 100, 567-578
3. Zhang, J-T. and Nicholson, BJ (194) J. Membrane Biol. 139, 15-29
4. Nishi M et al (1991) Dev. Biol. 146, 117-130; Kumar, NM (1986) J Cell Biol. 103, 767-776