Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot:1-10ug/ml
ELISA: 1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide:
C7854-05F: Connexin 32, Rat Control Peptide
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| A synthetic 19 aa peptide sequence within the 2nd cytoplasmic domain of the rat Cx32 (KLH). |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, before the addition of 40% glycerol. |
| Specificity: |
| Recognizes rat Cx32 (Connexin 32). Species sequence homology: mouse, human, guinea pig, bovine and ovine 100%. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Kumar, Nm and Giula, NB (1996) Cell 84, 381-388; White , WT et al (1995) Kidney Intl. 48, 1148-1157; Evans, HW (1994) Biochem. Soc. Tr. 788-792; Byer, E et al (1990) J. membrane Biol. 116, 187-194
2. Paul D (1986) J Cell Biol. 103, 123-134; Heynkes, R et al (1986) FEBS Lett. 205, 56-60; Nicholson, B (1985) JBC 260, 6514; Zimmer, DB et al (1987) JBC 262, 7751; Rahman, S et al (1991) J Cell Sci. 100, 567-578
3. Zhang, J-T. and Nicholson, BJ (194) J. Membrane Biol. 139, 15-29
4. Nishi M et al (1991) Dev. Biol. 146, 117-130; Kumar, NM (1986) J Cell Biol. 103, 767-776
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