		Technical Data
C7854-05G	Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)

Description:
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Dalton. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, Cx26-50. Hydropathy nalyses
of Cx sequences predicts 4 transmembrane TM, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N- terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng Cx32 control peptide/well.
Optimal dilutions to be determined by the researcher.

Control Peptide:
C7854-05J Connexin 32, Rat Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Mouse	Rabbit

Concentration:

Not determined

Immunogen:

A 19aa peptide sequence near the C-terminal cytoplasmic domain of mouse Cx32 (KLH).

Purity:

Serum

Form

Supplied as a liquid, 0.05% sodium azide, 40% glycerol.

Specificity:

Recognizes mouse Connexin 32. Species sequence homology: rat and human 90%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

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