Technical Data
C7854-05M
Connexin 32, aa105-123 (GJB1, CX32)
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Technical Data |
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C7854-05M |
Connexin 32, aa105-123 (GJB1, CX32) |
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Description: A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin 32 belongs to the class 1 group of connexins. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1.0ug/ml Immunohistochemistry (formalin/acetone fixed tissues): 1-2ug/ml Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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