Technical Data
C7854-05M
Connexin 32, aa105-123 (GJB1, CX32)
Description:
A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin 32 belongs to the class 1 group of connexins.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1.0ug/ml
Immunohistochemistry (formalin/acetone fixed tissues): 1-2ug/ml
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9G341Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Synthetic peptide (MLRLEGHGDPLHLEEVKRH) corresponding to aa105-123 from Connexin 32. Conjugated to KLH.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Specificity:
Recognizes human connexin-32, aa105-123. Species Crossreactivity: mouse, rat. Species sequence homology: chimpanzee, Cynomolgus Monkey, bovine, canine, guinea pig, hamster, chinese hamster, horse and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.