Technical Data
C7854-27A
Connexin 33 (CX33, Gap Junction Alpha 7 Protein, CXA-7)
Description:
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, CX26-50. Hydropathy analyses of CX sequences predicts 4 transmembrane domains, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using Chemiluminescence technique.

ELISA: 1:10,000-100,000 using 50-100ng control peptide per well. See C7854-27B.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A 17 amino acid peptide sequence within the C-terminal cytoplasmic domain of Rat CX33
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of 40% glycerol.
Specificity:
Recognizes rat CX33. Immunogenic peptide sequence is specific for CX33 and no significant homology is seen with other Connexins. Sequence homology of rat CX33 peptide with other species is not known.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, N.M. and Giula, N.B., Cell 84: 381-388 (1996). 2. White, W.T., et al., Kidney Intl. 48: 1148-1157 (1995). 3. Evans, H.W., Biochem. Soc. Tr. 22: 788-792 (1994). 4. Byer, E., et al., J. Membrane Biol. 116: 187-194 (1990). 5. Haefliger, J.A., et al., JBC 267: 2057-2064 (1992).