Technical Data
C7855-14
Connexin 36 (CX36)
Description:
Intercellular communication through gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation and growth control. Interestingly, reduction or alteration in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. The murine Cx36 gene encodes a protein of 321 amino acids most homologous to connexin 35. Based on the presence of an intron within its coding region the Cx36 gene is suggested to form a new delta subclass of murine connexin genes. Specific cell types in the brain express specific types of connexins; expression and expression patterns coincide with tissue compartmentalization and function. These compartments change during development. Cx36 is the first gap junction protein expressed predominantly in neuronal cells of the mammalian central nervous system. It is highly expressed in adult retina and is present in neurons of the inferior olive, the olfactory bulb, the CA3/CA4 hippocampal subfields and several brain-stem nuclei. Cx36 mRNA expression in the brain gradually increases during fetal development until day seven post-partum when its expression begins to decline. Biophysical measurements of gap junction channels formed by transfected and endogenous Cx36 indicate that they possess unique properties well-suited for mediating flexible electrical and biochemical interactions between neurons.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml; detects a single band at ~36kD.
Immunohistochemistry (Frozen): 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
Mouse Connexin 36 transfected HeLa cell lysates, mouse brain, retina and pancreas homogenates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminal region of the human Connexin 36 protein.
Purity:
Purified by epitope affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes mouse Connexin 36 on transfected HeLa cell lysates, mouse brain, retina and pancreas homogenates. The speci city of this antibody was demonstrated using Connexin 36 knockout mice. Species Sequence Homology: human, rat and zebra sh.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bennett, M.V., et al., Neuron 6(3): 305-320 (1991). 2. Condorelli, D.F., et al., Eur. J. Neurosci. 10(3): 1202-1208 (1998). 3. Kumar, N.M. & Gilula, N.B., Cell 84(3): 381-388 (1996). 4. Kuraoka, A., et al., J. Histochem. Cytochem. 41(7): 971-980 (1993). 5. Rosental, R., et al., Brain Res. Brain Res. Rev. 32(1): 11-15 (2000). 6. Sohl, G., et al., FEBS Lett 428(1-2): 27-31 (1998). 7. Sohl, G., et al., Eur. J. Cell Biol. 69(3): 267-275 (1996). 8. Srinivas, M., et al., J. Neurosci. 19(22): 9848-9855 (1999). 9. Wilgenbus, K.K., et al., Int. J. Cancer 51(4): 522-529 (1992).