Technical Data
C7855-15
Connexin 36 (CX36)
Description:
Intercellular communication through gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation, and growth control (1-4). Gap junctions are transmembrane channels that directly link neighboring cells by mediating the exchange of low-molecular weight (< 1200kD) metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. Connexons are hexameric assemblies of connexin proteins.
CX36 is the first gap junction protein expressed predominantly in neuronal cells of the mammalian central nervous system. It is highly expressed in adult retina and is present in neurons of the inferior olive, the olfactory bulb, the CA3/CA4 hippocampal subfields and several brain-stem nuclei (6). Cx36 mRNA expression in brain increases gradually during fetal development until day 7 post-partum, when its expression begins to decline (7). Biophysical measurements of gap junction channels formed by transfected and endogenous Cx36 indicate that they possess unique properties well suited for mediating flexible electrical and biochemical interactions between neurons (8). Recent studies have also shown that Connexin36 is essential for transmission of rod-mediated visual signals in the mammalian retina (10).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 5-10ug/IP reaction
Immunohistochemistry (Frozen): 1-3ug/ml
Optimal dilutions to be determined by the researcher.

positive control:
Human Connexin36-transfected HeLa cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G105Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceMouseMouse
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal region of the rat and mouse Connexin36 proteins. This sequence differs from the human Connexin36 sequence by one amino acid.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Speci c for the mouse ~36kD Connexin36 protein. Species Crossreactivity: Human. Species Sequence Homology: Rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, M. and Gilula, M.B., Cell 84: 381-388 (1996). 2. Saez, J.C., et al., In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al., Neuron 6: 305-320 (1990). 4. Kuraoka, A., et al., J. Histochem. and Cytochem. 41: 971-980 (1993). 5. Wilgenbus, KK., et al., Int. J. Cancer 51: 522-529 (1992). 6. Condorelli, D.F., et al., Eur. J. Neurosci. 10: 1202-1208 (1998). 7. Sohl, G., et al., FEBS Lett. 428: 27-31 (1998). 8. Srinivas, M., et al., J. Neurosci. 19(22): 9848-9855 (1999). 9. Nash, J.E., et al., PNAS 97(13): 7573-7578 (2000). 10. Deans, M.R., et al., Neuron 36(4): 703-712 (2002).