Technical Data
C7855-17
Connexin 36 (CX36)
Description:
Intercellular communication through gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation, and growth control. (14) Gap junctions are transmembrane channels that directly link neighboring cells by mediating the exchange of low-molecular weight (<1200kD) metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. Connexons are hexameric assemblies of connexin proteins. At least 14 connexin genes are known.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1.0ug/ml
Western Blot: 1ug/ml
Immunoprecipitation: 2-3ug/ IP
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.125mg/ml
Immunogen:
Peptide corresponding to a sequence from the C-terminus of rat and mouse Connexin 36 (Cx36). This sequence differs from the human Cx36 protein by a single amino acid.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes mouse Cx36 protein (36kD). Con rmed by Western blotting using rat retina and olfactory bulb. A band corresponding to Cx36 homodimers may be observed at ~66kD. Species Crossreactivity: rat and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kumar, M. and Gilula, M.B., Cell 84:381:388 (1996). 2. Saez, J.C., et al., In Advances in Second Messenger and Phosphoprotein Research; eds S., Shenolikar and A., Narin. Raven Press, New York (1993). 3. Bennet, M.V.L., et al., Neuron 6:305320 (1990). 4. Kuraoka, A., et al., J. Histochem. and Cytochem. 41:971980 (1993). 5. Wilgenbus, KK., et al., Int. J. Cancer 51:522529 (1992). 6. Condorelli DF, et al., Eur J Neurosci., 10:12021208 (1998). 7. Sohl G, et al., FEBS Lett., 428:2731 (1998). 8. Srinivas M, et al., J Neurosci 19(22):984855 (1999). 9. Nash, JE et al., PNAS 97(13) : 75737578 (2000). 10. Rash, JE, et al., J Neuroscience 21(6) : 19832000 (2001)