Technical Data
C7858-07A
Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)
Description:
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific. They allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junctions play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM and N-terminal CP domains are well conserved among family members. The Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml; Using ECL. The protein detected may migrate slightly faster than the 46kD.
ELISA: 1:100,000. Using 50-100ng C7858-07B Control Peptide/well.
Immunohistochemistry: 2-10ug/ml. Adherent cells can be fixed in 50% methanol-50% acetone or 1% paraformaldhyde.
Optimal dilutions to be determined by the researcher

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to 11aa within the C-terminal cytoplasmic domain of rat Cx46 conjugated to KLH. Species sequence homology: mouse, 91%; human, <50%.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat Cx46. No significant homology is seen with other Connexins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Banerjee, D. et al., (2010) Intl. J. Cancer 127; 839-848. 1. Fischer, R. (2005) Gastroenterology, 128; 433-448. 2. Laux-Fenton, W. (2003) Cornea. 22:457-464. 3. Jacobs, M.D., (2004), Invest. Ophthalmol.Vis.Sci, 45: 191-199. 4. Kumar, N.M. and Giula, N.B. (1996) Cell 84, 381-388; 5. White , W.T. et al (1995) Kidney Intl. 48, 1148-1157; 6. Evans, HW (1994) Biochem. Soc. Tr. 788-792; 7. Byer, E et al (1990) J. membrane Biol. 116, 187-194 8. Paul DL et al (1991) J Cell Biol. 115, 1077-1089. 9.. Zampighi, G.A. et al., (2005) Invest. Ophthalmol. Vis. Sci., 46: 3247. 10. Isakson, B.E. et al., (2001) Am J Physiol Cell Physiol 281: C1291. 11. Merriman-Smith, B.R.et al., (2003) Invest. Ophthalmol. Vis. Sci., 44: 3458 - 3466. 12. Yoshida, M. et al., (2005) Pathology Intl 55; 732-737.