Technical Data
C7865-19A
Contactin-associated protein 1 (Caspr1)
Description:
Contactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:300 detects Caspr in rat cerebellum and rat hippocampus tissues.
Western Blot: 0.5ug/ml detected Caspr on 10ug of rat brain membrane tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain membrane tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k11C235Affinity Purified
SizeStorageShippingSourceHost
100ug0CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to rat Caspr.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat Caspr. Species sequence homology: mouse 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wolswijk, G., et al. (2003). Brain. 126:1638- 1649. 2. Rios, J., et al. (2000). J. Neurosci. 20:8354. 3. Gollan, L., et al. (2003). J. Cell. Biol. 163:1213.